Web(2) Single-cell RNA sequencing of subcutaneous adipose tissues identifies therapeutic targets for cancer-associated lymphedema Cell Discovery was measured using the ImageJ software. Flow cytometry SVF was resuspended in cell staining buffer (420241, BioLegend), and then incubated with the following cellular surface antibodies at room … WebFOXP3 Fix/Perm Buffer Set - BioLegend's FOXP3 Fix/Perm Buffer Set possesses been specially formulates for intracellular staining FOXP3 with lowest efficacy to of appear fluorochrome staining.
Annexin V Binding Buffer - BioLegend
WebWash cells twice with cold BioLegend cell staining buffer (cat # 420241) and then resuspend cells in Annexin V Binding Buffer (cat # 422201) at a concentration of 1x10 6 cells/ml. 2. Transfer 100 µl of cell suspension in 5 ml test tube. 3. Add 5 µl of fluorochrome conjugated Annexin V. 4. Add 10 µl of PI solution (cat # 421301) or 7-AAD (cat ... WebRequest Bulk Quote. Description. Cell Staining Buffer is an antibody diluent and cell wash buffer optimized for use in immunofluorescent staining of viable or fixed single cell … hi heat spray foam
Protocol - Intracellular Flow Cytometry Staining Protocol
Web售价: ¥ 824.20 原价: ¥ 1268.00: 货号: 420241 : 品牌: Biolegend : 原产地: 分装/原装: 生产商 WebAnnexin V Binding Buffer - Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents. The buffer is provided as a ready-to-use solution.. WebMar 30, 2016 · Inhibitors and vehicle controls were added at the indicated times after which cells were washed with 500 uL of cell staining buffer from Biolegend ® (San Diego, CA, USA) (product #: 420241) and spun down at 2500 RPM for 3 min. Cells were resuspended in 100 uL of cell staining buffer prior to the addition of 5 uL of either PE mouse IgG1, κ ... hi hee shawhead