Hela vimentin

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August undefined, 2024

WebVimentin was one of the CCAT1 binding proteins and was participated in the tumor metastasis pathway. Knocked down VIM and rescued the downregulation by overexpressing CCAT1 demonstrated that CCAT1 could enhance tumor migration and invasion abilities by stabilizing Vimentin protein. Web(A) The expression of E-cadherin, N-cadherin and Vimentin in transfected HeLa cells was detected by Western blot. (B) The expression of E-cadherin, N-cadherin and Vimentin in transfected SiHa cells was detected by Western blot. Data were presented as mean ± standard deviation with three replicates.

Human Vimentin Profiling ELISA Kit [Colorimetric]

WebImmunogen Recombinant fragment within Human Vimentin (internal sequence). The exact sequence is proprietary. Database link: P08670 Positive control WB: HEK-293T, HeLa … WebAll lanes : Anti-Vimentin antibody [V9] - Cytoskeleton Marker at 1 µg/ml Lane 1 : U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate Lane 2 : Human tonsil cell lysate Lane 3 : Wild-type HeLa cell lysate Lane 4 : VIM knockout HeLa cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) … hull city council payment for rent

Rabbit mAb #5741 - Cell Signaling Technology

WebJun 26, 2024 · HeLa cells were transfected with NOX2 siRNA or NOX4 siRNA. After 24 h, cells were seeded over the electrode wells (b), six-well plate (c), and transwell plates (d). (b) HeLa cell growth curve in the presence of NOX2 or NOX4. NOX2 or NOX4 knockdown effect was assessed by measuring the change in HeLa cell growth responses using TGF … WebHeLa cell lysate was probed with anti-vimentin (clone RV202, Cat. No. 550513) at concentrations of 120 (lane 1), 60 (lane 2) and 30 ng/ml (lane 3). Vimentin is identified as a band of ~57 kDa. Show More Spectrum Viewer Protocol Library Scientific Resources Catalog No: Your Price: Sign In Unit: Size: 50 µg Request Bulk Reagent Quote Product … http://www.ab-mart.com.cn/page.aspx?node=%2077%20&id=%2053980 hull city council pay

Vimentin Monoclonal Antibody (J144) (MA3-745)

Vimentin phosphorylation and assembly are regulated by …

Vimentin was one of the CCAT1 binding proteins and was participated in the tumor metastasis pathway. Knocked down VIM and rescued the downregulation by overexpressing CCAT1 demonstrated that CCAT1 could enhance tumor migration and invasion abilities by stabilizing Vimentin protein. WebAll lanes : Anti-Vimentin antibody [V9] - Cytoskeleton Marker at 1 µg/ml Lane 1 : U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate Lane 2 : Human tonsil cell … hull city council pay riseWebAfter being transfected and screened with puromycin, it was found that compared with the HeLa cell controls, both mRNA level and protein expression of vimentin in the transfected HeLa cells were significantly decreased ( Figure 1 B,C), and vimentin knocked out cells were named KO-vimentin HeLa cells, abbreviated as KO HeLa cells. holiday parks near bristol uk

"WebHeLa cell lysate was probed with anti-vimentin (clone RV202, Cat. No. 550513) at concentrations of 120 (lane 1), 60 (lane 2) and 30 ng/ml (lane 3). Vimentin is identified … " - Hela vimentin

Hela vimentin

Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] (ab154207) - Abcam

WebMar 2, 2024 · Moreover, in CHIR-treated HeLa cells transiently expressing vimentin BC2T, the nanobody signal was almost exclusively detectable at vimentin fibers (Supplementary Fig. 2c, right panel). Web廖昱婷碩士論文，分析HURP p725/Vimentin複合體如何調控Golgi complex的形狀與定位，國立暨南國際大學應用化學系生醫碩士班，2024 鄭巧筠碩士論文，Poh1、Vimentin p72和HURP p725形成蛋白質複合體，並且調控MG132所誘導的aggresome形成，國立暨南國際大學應用化學系生醫碩士 ...

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WebVimentin protein expression levels were investigated by comparing the integrated intensity of spots visualized in 2-D PAGE gels of various cancers. Pancreatic tumor tissues … WebThe absence of signal in the vimentin knockout HeLa cells confirms specificity of the antibody for vimentin. Western blot analysis of extracts from various cell lines using …

WebJun 22, 2001 · As shown in Figure 4, vimentin in untreated, control HeLa cells (left-hand, upper panel) was organized into an extensive network of cytoplasmic intermediate filaments. However, after 3 h of ... WebVimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of …

WebHuman VIM Knockout HeLa Cell Lysate (CAT#: NCL2008ZP279) Neural Antibodies. Mouse Anti-VIM Monoclonal Antibody (3H9D1), Unconjugated (CAT#: NAB20101743CR) Rabbit Vimentin Antibody (CAT#: NAB-2102-MP158) ... Thank you for your interest in Human Vimentin Profiling ELISA Kit [Colorimetric]. Please provide us with your contact … WebMay 1, 2009 · The surface vimentin expression pattern observed in HeLa cells is virtually identical to confocal observation of surface vimentin expression previously reported in macrophages . Fixation and staining procedures to verify cell surface and cytosolic staining were verified through the ability to detect β-COP, an intra-Golgi transport marker ...

WebSep 20, 2013 · For the establishment of the HeLa/endoB2-St and HeLa/vimentin-GFP stable cell lines, cells were transfected as for transient transfections and grown for 48 h in culture medium without G418. After trypsinization and appropriate dilutions, cells were grown for 3 weeks in culture medium supplemented with 0.8 mg/ml G418, and single colonies …

WebThis study represents a comprehensive description of a HeLa xenograft model for in vivo investigations, enabling researchers to assess new treatments for cervical cancer. ... (CK), vimentin, P53, CD34, and Ki-67. Results: Tumor take rate, mean doubling time and latency period were 94.4%, 5.29 ± 3.57 days and 15.27 days, respectively. H&E ... holiday parks near bridport dorset holiday parks near bracknellWebMar 31, 2024 · At 8 hpi, cells were fixed, nuclei and replication sites were stained with Hoechst and immunostaining was performed to visualise vimentin. In untreated HeLa cells, vimentin was associated with replication sites and newly assembled virions were found throughout the cytoplasm (Figure 3e). As expected, in acrylamide-treated HeLa cells … hull city council parking enforcementWebDec 1, 1992 · Using both vimentin-expressing (HeLa) and vimentin-negative (MCF-7) epithelial cell lines, we have identified the cis-regulatory DNA elements involved in this process. Sequences located 1.1-0.6 kb upstream of the vimentin transcription-initiation site strongly enhance expression in HeLa cells, but are silenced in MCF-7 cells. hull city council pay grades april 2021WebThis study represents a comprehensive description of a HeLa xenograft model for in vivo investigations, enabling researchers to assess new treatments for cervical cancer. ... hull city council pay scales 2023WebAug 21, 2015 · Vimentin, the major intermediate filament of mesenchymal cells, is mainly involved in tissue integrity and cytoarchitecture 1. The evolutionarily highly conserved protein consists of a central... holiday parks near butlinsWebThe absence of signal in the Vimentin knockout HeLa cells confirms specificity of the antibody for Vimentin. Show More Simple Western™ analysis of lysates (1 mg/mL) from … holiday parks near cheddar gorge