Peasy t3
WebpEASY®- T3 Cloning Vector provides dual- Eco RI and dual- Not I restriction sites. It is designed for cloning and sequencing Taq- amplified PCR products 3’A-overhangs). The cloned insert can be released from a single enzyme digestion. The vector is suitable for T7 RNA polymerase-mediated In vitro transcription using our T7 High-Efficiency ... WebpEASY® -T3 Cloning Kit 目录号： CT301-01 单 价： ￥620 规格： 20 rxns 60 rxns 数量： - + 说明书 载体信息 质检报告 立即下单 加入购物车 产品详情介绍 pEASY®-T3 Cloning …
Peasy t3
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WebMay 29, 2024 · Therefore, pEASY T3-ydhE was used for the positive control of a H + -coupled norfloxacin efflux transporter, whereas pEASY T3-nhaD was used for the positive … WebApr 21, 2024 · The white rot fungus Irpex lacteus is one of the most potent fungi in degradation of lignocellulose and xenobiotics. Two manganese peroxidases ( Il MnP1 and Il MnP2) from I. lacteus CD2 were over-expressed in Escherichia coli and successfully refolded from inclusion bodies. Both Il MnP1 and Il MnP2 oxidized the phenolic compounds …
WebThe pEASY-T3 Cloning Kit was used for ligating the PCR product with the T3 cloning vector. The ligation product was transformed into Escherichia coli and transferred to Comate Bioscience Co., Ltd for sequencing. The full-length TaEXPA9-A/B/D sequences were analyzed using the method from Peng and Feng[4, 5]. Pa ge 5/ 24 Web将稳定表达pCPK6∷GUS的拟南芥T3转基因种子播于全营养培养基(CK)，4 ℃低温处理3 d，竖直光照培养7 d，分别移至CK和Ca2+、Fe2+、Mg2+、Zn2+和Mn2+缺乏的固体培养基上。继续竖直光照培养7 d后，采用组织化学法定位检测GUS活性。
WebNov 12, 2013 · The amplified fragments were subsequently purified using the EasyPure Quick Gel Extraction Kit (TransGen) and cloned into pEASY-T3 plasmid using the pEASY T3 Cloning kit (TransGen) according to the manufacturer's recommendations. To identify sequencing errors, at least three clones of each sample were sequenced (SunbioTech).
WebJan 11, 2024 · For the attempts to delete the pfkA, pfkB and zwf genes in E. coli MG1655(DE3), the J23119 promoter fused with a 20-nt guiding sequence (pfkA: GTGTCTGACATGATCAACCG, pfkB: CACGTACATGTGGAAGCAAG, zwf: GCGTGCTGACTGGGATAAAG) was integrated into pEASY-T3 via TA cloning, and then the …
WebTriiodothyronine, also known as T3, is one of the two main hormones your thyroid gland releases into your bloodstream. Your thyroid also produces thyroxine, also known as T4 … h r essentials ltdWebA T3 (triiodothyronine) test helps diagnose thyroid conditions, particularly hyperthyroidism (overactive thyroid). Your thyroid is a small, butterfly-shaped gland located at the front of your neck under your skin. It’s a part of your endocrine system. Triiodothyronine, also known as T3, is one of the two main thyroid hormones. hr essex organizerWebSep 6, 2024 · pEASY-Blunt Cloning vector to obtain the recombinants, and then transformed the recombinants into ... The PCR product of BtabOBP3 was cloned into a pEASY-T3 vector, and then the target fragment ... hoag hospital newport beach human resourcesWeb15 hours ago · Rejuvenating your leggy and stretched-out succulents is so easy. 1. Remove the whole plant from its pot. 2. Carefully twist off any dead or dying leaves. 3. Continue by … hress login arWebThe pEASY-T3 vector (TransGen, Beijing, China) was used for plasmid gene cloning and sequencing. The plasmid pET-28a (+) (Takara Bio, Otsu, Japan) was used as an expression vector. hres schoolpEASY® -T3 Cloning Kit Catalog Number：CT301-01 Price：$89.02 Specification： 20 rxns 60 rxns Quantity： Manual Vector Information MSDS COA Inquire Now Buy Now Add to Cart Free Sample Avaliable Product Details pEASY® - T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. hresko architectsWebpEASY®-Basic Seamless Cloning and Assembly Kit Products PCR, RT-PCR, qPCR, qRT-PCR Restriction/Modifying Enzymes DNA Molecular Weight Standards Cloning and … hoag hospital newport beach ca npi