Phis2 载体

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August undefined, 2024

Webb14 aug. 2015 · 伟通生物汇集了数以百计的克隆载体，包含常规原核表达载体、昆虫细胞表达载体、哺乳动物表达载体、酵母表达载体、慢病毒表达载体、腺病毒表达载体、腺相关病毒表达载体、逆转录病毒表达载体、信号通道报告系统等。 Webb根据pHIS2载体上Eco R Ⅰ和Sac Ⅰ限制性内切酶识别位点的前后序列设计载体引物，进行PCR菌液检测，以阳性菌液为模板的PCR扩增的DNA条带要比以pHIS2空载体为模板的PCR扩增的条带分子量大20个碱基左右。

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http://www.honorgene.com/product_list/carrier_library/123112.html Webb质粒简介：. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene,located downstream of a multiple ... software m221

pHIS2载体_质粒图谱 - 优宝生物

WebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the … http://www.biofeng.com/zaiti/jiaomu/pAbAi.html software m601

Phis2 载体

WebbClose You have no items in your shopping cart.. Home; Plasmid; p53- His2 Model: PVT11251 WebbATCC菌种库 ATCC细胞库普通细胞株其他菌种基因cDNA质粒表达质粒 Add基因质粒和载体感受态细胞大肠杆菌表达载体哺乳细胞表达载体酵母表达载体昆虫表达载体腺病毒载体慢病毒载体逆病毒载体 Gateway载体植物表达载体 Crispr/Cas9载体广宿主载体无细胞 …

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WebbpHIS2 载体出品公司 : 质粒类型 : 克隆方法 : 载体大小 : 载体标签 : 载体抗性 : 备注 : Clontech 酵母单杂交系统多克隆位点，限制性内切酶 7207bp (查看载体序列) TRP1, HIS3 Kanamycin (卡那霉素) Clone in cis-acting sequences for use with 1-hybrid system Webb26 maj 2024 · 载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene, located downstream of a …

Webb26 maj 2024 · 载体描述 pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically … Webb扩增从裂殖酵母基因获得的高柠檬酸合酶基因，然后转移到载体pHIS2上，这个载体在氮末端有his6 标签，并包含烟草花叶病毒蛋白酶裂解位点。突变体通过QuikChange直接位点突变工具包实现突变，并由DNA测序予以确定。

WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog No. 630304 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ... Webb使用pHIS2载体，将启动子序列构建在HIS3报告基因前面的多克隆位点上，对应的转录因子构建在AD载体上即可。使用Y187菌株，它相对于AH109菌株在基因组上少了HIS的报告 …

WebbpHIS-2 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available …

Webb11 juli 2014 · 常用载体及通用测序引物.docx 2014-07-11 上传常用载体及通用测序引物,测序通用引物,华大测序通用引物,载体通用引物,petduet 1测序引物,通用引物,测序引物,pet28a测序引物,m13通用引物,细菌通用引物,病毒通用引物 slow implementationhttp://www.zoonbio.com/molecular/yeast-one-hybrid-procedure.html slow image receptorWebb20 juni 2024 · 产品详细描述载体基本信息 Clontech pHis2 酵母单杂交载体 -- -- 多克隆位点，限制性内切酶 7207bp -- -- -- 卡纳霉素 -- 含HIS3报告基因 -- -- -- -- 载体质粒图谱和多克 … software m7Webb16 mars 2024 · 诱饵载体：pHis2-DNA（筛选标记Trp报告基因His）文库载体：pGADT7-cDNA（筛选标记Leu）实验步骤：一、转化诱饵载体（参考SK2400中质粒转化步骤） … slow imac performance snow leopardhttp://www.biofeng.com/zaiti/jiaomu/pHis2.html software m711Webb1、提供详细的、完整的实验报告，清晰的实验图片. 2、在受体菌的阳性菌. 自激活和毒性验证. 1、pHis2-Bait或pAbAi-Bait质粒. 2、或者Bait序列，质粒由我们构建. 酵母质粒提取和测序. 酵母菌液或者具有菌斑的平板. 1、提供详细的、完整的实验报告. 2、筛选结果的测序 ... software m711 cobrahttp://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html slow imperial march