Phis2自激活

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August undefined, 2024

Webb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, 0.5 μL of PCR products (Y1 + Y3 and Y2 + Y3), 1 μL of 10× ligation buffer, 3 U of T4 ligase, and PEG6000 at final concentration of 10% (w/v). http://www.zoonbio.com/molecular/yeast-one-hybrid-procedure.html

pHIS2 - lifescience-market.com

WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ... diagnosis code for failed back surgery

pHIS2 - lifescience-market.com

http://jushengwu.com/jbk-53/ Webb9 juni 2024 · 南京瑞源生物技术有限公司主要提供酵母膜蛋白、酵母文库、酵母菌落、酵母单杂、酵母双杂、核转录因子、启动子结合、逆境筛选、分子互作等解决方案及技术服务，业务范围覆盖江苏、浙江、山东、河南、陕西、福建、北京、上海等地。 http://www.kelei-biology.com/plus/view.php?aid=1071 cingal benefits

p53-His2 plasmid,p53-His2,p53-His2 plasmid,p53-His2 sequence,p53-His2 …

如何利用生信预测两个蛋白是否互作？ - 知乎

WebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. WebbpHIS2-p53+pGADT7-53m（阳性对照） pHIS2+pGADT7-53m（阴性对照）服务特色：经验足周期短性价比高通量高. 客户提供. 构建酵母文库材料. 细胞样本：细胞数量＞ 1*10^7 动物样本：＞lg . 植物样本：＞ 2g 总RNA: ＞200μg. 酵母单杂筛库材料. 启动子序列含有诱饵 … cingal fcWebb19 nov. 2012 · 是选取atg上游2000的片段，然后整个连到phis2载体上吗？我之前做了直接取ATG上游1.5k构建到phis2上，感觉不合适。最好还是找到自己转录因子的结合motif … diagnosis code for fatigue and weakness

"Webb使用phis2载体，将启动子序列构建在his3报告基因前面的多克隆位点上，对应的转录因子构建在ad载体上即可。使用y187菌株，它相对于ah109菌株在基因组上少了his的报告基 … " - Phis2自激活

Phis2自激活

http://baiyk.com.cn/ProDetail.aspx?ProID=56 WebbY187-pHis2 Yeast One-Hybrid Media Kit（酵母单杂培养基套装YM1010），含有用于Y187-pHis2系统酵母单杂交文库筛选的全套培养基，性价比更高。. 培养基以非常方便使用的铝箔袋形式包装，每个独立包装可以配制0.5 L的培养基。. 使用时仅需要将袋中的所有干粉倒入 …

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Webb诱饵载体：pHis2-DNA(筛选标记Trp报告基因His) 文库载体：pGADT7-cDNA(筛选标记Leu) 实验步骤：一、转化诱饵载体(参考KD0256中质粒转化步骤) 1.YPDA平板培养划线，活化Y187酵母菌种；挑酵母菌落于液体YPDA培养基摇菌培养； 2.制备感受态并将pHis2-DNA转化进入感受态细胞； Webb5 nov. 2015 · 酵母双杂交 AH109,Y187. 下面所有质粒和菌株，均为本公司实验室亲自验证过，可以提供，载体来源于 promega,addgene,clontech,novagen,invitrogen 可提供质粒图谱，可提供近万种质粒载体、菌株、基因和细胞株, 并可提供实验技术外包服务, 如基因克隆、质粒构建、蛋白原核 ...

Webb产品参数质粒名称：pHis2 出品公司：Clontech 质粒类型：酵母单杂交系统克隆方法：多克隆位点，限制性内切酶载体大小： 7207bp 原核抗性： Kanamycin (卡那霉素) 真核抗 … http://www.zoonbio.com/molecular/yeast-one-hybrid.html

http://www.qiyuebio.com/product/61298 Webb服务时间：24小时在线客服 . 客服热线：400-000-7085

Webb22 juli 2013 · Description. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit （#K1617-1）. It contains a HIS3 nutritional reporter gene, located downstream of a …

Webbactivation domain in pGADT7-Rec2. The target DNA sequence, or Bait Sequence, is cloned into pHIS2.1 as one copy or tandem repeats. Interaction between a DNA-binding protein … diagnosis code for family therapyhttp://www.youbio.cn/product/vt1637 cingal couponWebbpHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication se-quence ( ARS4) and TRP1 nutritional marker for replication and selection in yeast (1, … diagnosis code for feeding tubehttp://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html diagnosis code for folate testingWebb载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit. It contains a HIS3 nutritional reporter gene, located downstream of a multiple cloning ... diagnosis code for fibrocystic diseaseWebb质粒简介：. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene,located downstream of a multiple ... cingal injection genouWebb酵母是单细胞低等的真核生物，具有生长快、成本低、易于遗传操作，能对外源蛋白进行翻译后加工和修饰、不产生有毒产物等特点，认为是外源蛋白的合适宿主 [1] 。酵母分为三类：第一是酿酒酵母；第二是栗酒裂殖酵母；第三是非常规酵母，主要有巴氏毕赤酵母、乳酸克鲁维亚酵母等 [2] 。 diagnosis code for finger injury